MeRIP-seq 探究沙门菌感染对鸡肝脏转录组 RNA m6A 修饰的影响
廖祖蓉
(
江西农业大学
, 江西 , 南昌 330045
)
粟永春
(
广西金陵农牧集团有限公司
, 广西 , 南宁 530049
)
吴东晓
(
江西农业大学
, 江西 , 南昌 330045
)
丁振玄
(
江西农业大学
, 江西 , 南昌 330045
)
刘浩东
(
江西农业大学
, 江西 , 南昌 330045
)
黄旭雯
(
江西农业大学
, 江西 , 南昌 330045
)
王文豪
(
江西农业大学
, 江西 , 南昌 330045
)
魏文华
(
江西农业大学
, 江西 , 南昌 330045
)
潘 航
(
江西农业大学
, 江西 , 南昌 330045
)
吴欢生
(
江西农业大学
, 江西 , 南昌 330045
)
陈 彪
(
广西金陵农牧集团有限公司;江西农业大学
, 广西 , 南宁 530049
)
陈智武
(
广西金陵农牧集团有限公司
, 广西 , 南宁 530049
)
黄 超
(
广西金陵农牧集团有限公司
, 广西 , 南宁 530049
)
中图分类号:S831.2
文献标志码:A
文章编号:1008-3847(2026)04-0024-09
基金项目：农业生物育种重大项目（2023ZD0405302）；国家重点研发计划“农业生物种质资源挖掘与创新利用”重点专项资助（2021YFD1200803）。
作者简介：廖祖蓉，硕士研究生，畜牧；粟永春，高级畜牧师，畜牧兽医生产研究。
通讯作者：陈智武，研究员，家禽遗传育种研究；黄超，高级畜牧师，畜牧兽医生产研究。
摘要:
沙门菌影响着全球家禽产业的发展，其感染鸡以及后续鸡的免疫反应机制较复杂。本研究以商品代白羽肉鸡为研究对象，采用江西农业大学分离的沙门菌菌株，在 3 日龄时通过灌胃的方式每只雏鸡接种 3×107CFUs 沙门菌悬液，对照组灌服相同剂量的磷酸盐缓冲液（PBS）。灌胃 14 d 后采集实验组和对照组的肝脏组织，进行转录组 MeRIP-seq 及 input RNA-seq 分析。结果表明，沙门菌感染鸡后肝脏
转录组 m6A 修饰特征（包括 peak 在基因区域的分布、motif 序列等）没有显著变化，但 m6A 修饰图谱有明显改变。在P < 0.05 和差异倍数大于 2 条件下，肝脏转录组中有 182 个 m6A peaks 的修饰程度在沙门菌攻毒组中显著加强，1 352 个 peaks 显著减弱。Input RNA-seq 数据表明，在 FDR< 0.05 和差异倍数大于 2的条件下， 实验组与对照组相比有 1 429 个基因表达显著上调， 729 个基因表达显著下调。富集分析结果表明，差异 peaks 关联基因显著富集到与物质代谢、机体免疫相关通路上。两组学联合分析表明，免疫相关基因 NFKBIA、IL16、CD5、IL21R 和 STAT4 等均呈 m6A 修饰程度降低而表达量升高模式，说明沙门菌感染后肝脏转录组 m6A 修饰广泛参与了鸡免疫反应过程。上述结果为进一步探究 RNA m6A 修饰调控鸡免疫反应奠定基础。
关键字:
鸡 沙门菌 肝脏 转录组 RNA N6-甲基腺苷
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